mouse anti sv2 igg antibody (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank
mouse anti sv2 igg antibody
Mouse Anti Sv2 Igg Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+sv2+igg/anti-Synaptic+vesicle+glycoprotein+2A/pmc12765181-2-0-7
Average 97 stars, based on 110 article reviews
Mouse Anti Sv2 Igg Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+sv2+igg/anti-Synaptic+vesicle+glycoprotein+2A/pmc12765181-2-0-7
Average 97 stars, based on 110 article reviews
mouse anti sv2 igg antibody - by Bioz Stars,
2026-09
97/100 stars
Images
1) Product Images from "Protocol for applying expansion microscopy to the study of mammalian neuromuscular junctions"
Article Title: Protocol for applying expansion microscopy to the study of mammalian neuromuscular junctions
Journal: STAR Protocols
doi: 10.1016/j.xpro.2025.104272
Figure Legend Snippet: Data analysis methods of expanded NMJs (A) Example of an expanded human NMJ stained with α-Bungarotoxin to label AChRs (cyan) and immunolabeled for SV2 to visualize presynaptic vesicles (magenta). Scale bar= 4 μm. A zoomed-in region (i) illustrates the selected area for measurement. AChR width was quantified on thresholded images (ii), indicated by red bars. AChR strip length and inter-strip spacing were measured on skeletonized images (iii), with yellow bars showing strip length and blue bars indicating spacing. Scale bar in (i)= 2 μm. (B) Mouse NMJ labeled as in (A). The yellow-dotted rectangle denotes the region used for fluorescence intensity profile analysis of AChRs and SV2. Intensity profiles were generated separately for each channel. Scale bar= 4 μm. Scale bar corresponds to the physical size of the expanded sample (not adjusted to expansion factor).
Techniques Used: Staining, Immunolabeling, Stripping Membranes, Labeling, Fluorescence, Generated
Related Articles
other:Article Title: Protocol for applying expansion microscopy to the study of mammalian neuromuscular junctions. Article Snippet: Antibody Incubation, labeling and Biomolecule Anchoring: a. AChR Labeling: Use Alexa-488 α-Bungarotoxin (α-BTX, Alexa Fluor 488 conjugate, B13422, Invitrogen) to label the AChRs. Article Title: Expansion microscopy reveals nano-scale insights into the human neuromuscular junction Article Snippet: Incubation:Article Title: Expansion microscopy reveals nano-scale insights into the human neuromuscular junction Article Snippet: Subsequent permeabilisation with Triton X-(4%) was applied to facilitate antibody penetration, whilst a blocking solution consisting of 4% of Bovine serum albumin (BSA) (w/v) and 2% (v/v) Triton X-(Sigma-Aldrich, UK) was applied to prevent non-specific binding. .. Muscle bundles were then subjected to overnight incubation at 4°C with a primary antibody, either Article Title: Terminal Schwann cells at the human neuromuscular junction Article Snippet: .. Muscle fibres were placed in the following sequence of solutions (made up in 1× PBS unless otherwise specified): glycine for 15 min to reduce tissue auto-fluorescence; 15 min wash in PBS; tetramethyl-rhodamine isothiocyanate-conjugated α-bungarotoxin (TRITC α-BTX; BTIU00012, VWR International Ltd ) 2 μg/ml for 15 min to label AChRs; 4% Triton X-100 for 1.5 h for permeabilization; a blocking solution of 4% bovine serum albumin (BSA) and 2% Triton X-100 for 30 min. Tissue was then incubated with the following primary antibodies overnight at room temperature: ready-to-use rabbit polyclonal anti-S100 IgG (Dako Omnis) which labels S100b strongly, S100A1 weakly, and S100A6 very weakly; mouse anti-S100 antibody (in BSA at 1:100 dilution, ab7852, Abcam); and rabbit monoclonal anti NG2 IgG (neuron-glia protein 2; in BSA at 1:100 dilution, ab255811, Abcam) which labels tSCs; Article Title: Expansion microscopy reveals nano-scale insights into the human neuromuscular junction. Article Snippet: Subsequent permeabilisation with Triton X-(4%) was applied to facilitate antibody penetration, whilst a blocking solution consisting of 4% of Bovine serum albumin (BSA) (w/v) and 2% (v/v) Triton X-(Sigma-Aldrich, UK) was applied to prevent non-specific binding. .. Muscle bundles were then subjected to overnight incubation at 4◦C with a primary antibody, either Sequencing:Article Title: Terminal Schwann cells at the human neuromuscular junction Article Snippet: .. Muscle fibres were placed in the following sequence of solutions (made up in 1× PBS unless otherwise specified): glycine for 15 min to reduce tissue auto-fluorescence; 15 min wash in PBS; tetramethyl-rhodamine isothiocyanate-conjugated α-bungarotoxin (TRITC α-BTX; BTIU00012, VWR International Ltd ) 2 μg/ml for 15 min to label AChRs; 4% Triton X-100 for 1.5 h for permeabilization; a blocking solution of 4% bovine serum albumin (BSA) and 2% Triton X-100 for 30 min. Tissue was then incubated with the following primary antibodies overnight at room temperature: ready-to-use rabbit polyclonal anti-S100 IgG (Dako Omnis) which labels S100b strongly, S100A1 weakly, and S100A6 very weakly; mouse anti-S100 antibody (in BSA at 1:100 dilution, ab7852, Abcam); and rabbit monoclonal anti NG2 IgG (neuron-glia protein 2; in BSA at 1:100 dilution, ab255811, Abcam) which labels tSCs; Blocking Assay:Article Title: Terminal Schwann cells at the human neuromuscular junction Article Snippet: .. Muscle fibres were placed in the following sequence of solutions (made up in 1× PBS unless otherwise specified): glycine for 15 min to reduce tissue auto-fluorescence; 15 min wash in PBS; tetramethyl-rhodamine isothiocyanate-conjugated α-bungarotoxin (TRITC α-BTX; BTIU00012, VWR International Ltd ) 2 μg/ml for 15 min to label AChRs; 4% Triton X-100 for 1.5 h for permeabilization; a blocking solution of 4% bovine serum albumin (BSA) and 2% Triton X-100 for 30 min. Tissue was then incubated with the following primary antibodies overnight at room temperature: ready-to-use rabbit polyclonal anti-S100 IgG (Dako Omnis) which labels S100b strongly, S100A1 weakly, and S100A6 very weakly; mouse anti-S100 antibody (in BSA at 1:100 dilution, ab7852, Abcam); and rabbit monoclonal anti NG2 IgG (neuron-glia protein 2; in BSA at 1:100 dilution, ab255811, Abcam) which labels tSCs; |
